SAFETY DATA SHEET
Trypan Blue
1. IDENTIFICATION
Product Identifiers
Product Name:
Trypan Blue
Other Names:
C.I. 23850; Direct Blue 14; Niagara Blue 3B.
Product Number(s):
C091, C0915
CAS Number:
72-57-1
Recommended use of the chemical and restriction on use
Laboratory use.
Biological Stain.
Company Details
ProSciTech Pty Ltd
11 Carlton Street
KIRWAN QLD 4817
Australia

(07) 4773 9444
www.proscitech.com
Emergency Contact Details
ProSciTech Pty Ltd
11 Carlton Street
KIRWAN QLD 4817
Australia

(07) 4773 9444
www.proscitech.com
2. HAZARDS IDENTIFICATION
Classification of the substance or mixture
Carcinogenicity (Category 2)
Label Elements
Signal Words
Danger
Hazard Statement(s)
H350: May cause cancer
Precautionary Statement(s)
P201: obtain special instructions before use.
P202: Do not handle until all safety precautions have been read and understood.
P405: Store locked up.
P281: Use PPE as required.
P308 +P313: If exposed or concerned: contact doctor/physician.
P501: Dispose of contents/container to an approved waste disposal plant.
Primary route(s) of entry
Not available.
Human Health
Inhalation:
Harmful by inhalation.
Ingestion:
Harmful if swallowed.
Eyes:
Risk of serious damage to eyes.
Skin:
Harmful in contact with skin.
Environment
Not available.
3. COMPOSITION/INFORMATION ON INGREDIENTS
Name
CAS No.
Content (w/w)
Classification
Tetrasodium 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)] bis[5-amino-4-hydroxynaphthalene
72-57-1
100%
-
4. FIRST AID MEASURES
Ingestion
Do not induce vomiting without medical advice. Rinse with water. Consult physician.
Inhalation
Move to fresh air. Consult a physician.
Skin Contact
Rinse with plenty of soap & water and seek medical advice.
Eye Contact
Rinse immediately with plenty of water and seek medical advice.
Other Information
Treat symptomatically and supportively.
5. FIREFIGHTING MEASURES
Suitable extinguishing equipment
Use water spray, dry chemical, carbon dioxide, or alcohol-resistant foam.
HAZCHEM
Not available.
Special protective equipment and precautions for fire fighters
As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion.
6. ACCIDENTAL RELEASE MEASURES
Personal precautions, protective equipment and emergency procedures
Use proper PPE as indicated in Section 8.
Environmental precautions
Avoid runoff into storm sewers and ditches which lead to waterways.
Do not let product enter drains.
Prevent further leakage or spill is safe to do so.
Methods and materials for containment and clean up
Clean up spills immediately, observing precautions in the Protective Equipment section. Sweep up, then place into a suitable container for disposal. Avoid generating dusty conditions. Provide ventilation.
7. HANDLING AND STORAGE
Precautions for safe handling
Wash thoroughly after handling. Wash hands before eating. Remove contaminated clothing and wash before reuse. Use with adequate ventilation. Avoid contact with eyes, skin, and clothing. Keep container closed when not in use. Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances.
Conditions for safe storage
Keep container closed when not in use. Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances.
8. EXPOSURE CONTROLS/PERSONAL PROTECTION
Exposure Standards
Engineering controls
Eyebath/eyewash & Safety shower.
Use process enclosure, local exhaust ventilation, or other engineering controls to control airborne levels.
Personal protective equipment
Eye and face protection
Safety glasses.
Skin protection
The selected protective gloves have to satisfy the specifications of EU Directive 89/686/EEC and the standard EN 374 derived from it. Handle with gloves. 0.11mm thick nitrile.
Body protection
Choose body protection according to the amount and concentration of the dangerous substance at the work place.
Hygiene measures:
Avoid contact with skin, eyes and clothing. Wash hands before breaks and immediately after handling the product.
Respiratory protection
Where risk assessment shows air-purifying respirators are appropriate use a full-face particle respirator type N99 (US) or type P2 (EN 143) respirator cartridges as a backup to engineering controls. If the respirator is the sole means of protection, use a full-face supplied air respirator. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN.
9. PHYSICAL AND CHEMICAL PROPERTIES
General information
Appearance
Dark blue to black powder.
Odour
Ordorless.
pH
Not available.
Vapour Pressure
Not available.
Density
Not available.
Boiling Point
Not available.
Melting Point
>300°C (>572°F)
Solubility
Not available.
Specific Gravity of Density
Not available.
Flash Point
Not available.
Flammable (Explosive) Limits
Not available.
Ignition Temperature
Not available.
Formula
C34H24N6Na4O14S4
10. STABILITY AND REACTIVITY
Reactivity
No further relevant information available.
Chemical stability
Stable under normal temperatures and pressures.
Possibility of hazardous reactions
No further relevant information available.
Conditions to avoid
Incompatible materials, dust generation, excess heat, strong oxidants.
Incompatible materials
Oxidizing agents.
11. TOXICOLOGICAL INFORMATION
Acute effects
No further relevant information available.
Eye contact
No further relevant information available.
Skin contact
No further relevant information available.
Ingestion
No further relevant information available.
Inhalation
No further relevant information available.
Toxicity and irritation
LD50/LC50:
CAS# 72-57-1:
Oral, rat: LD50 = 6.200 mg/kg;
12. ECOLOGICAL INFORMATION
Ecotoxicity
LC50-Oryzias latipes >1,000mg/l-48h
Persistence and degradability
No further relevant information available.
Bioaccumulative potential
No further relevant information available.
Other adverse effects
No further relevant information available.
13. DISPOSAL CONSIDERATIONS
General information
Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification. Contact a licensed professional waste disposal service to dispose of this material.
14. TRANSPORT INFORMATION
ADG label required
HAZCHEM
Not available.

UN Number
UN307
Proper shipping name
(Tetrasodium 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis[5-amino-4-hydroxynaphthalene-2,7-disulphonate])
Transport hazard class
Void
Packing group
None.
Environmental hazard
Yes
Special precautions for users
Not regulated.
Additional information
No information available.
15. REGULATORY INFORMATION
Poisons Schedule Number
Not listed.
Other Information
No further relevant information available.
16. OTHER INFORMATION
SDS preparation date
7 October 2021
Comments
This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and shall not establish a legally valid contractual relationship.
This Safety Data Sheet (SDS) has been prepared in compliance with the Preparation of Safety Data Sheets for Hazardous Chemicals Code of Practice February 2016. It is the user's responsibility to determine the suitability of this information for adoption of necessary safety precautions. The information published in this SDS has been compiled from the publications listed in Section 16: to the best of our ability and knowledge these publications are considered accurate. We reserve the right to revise Safety Data Sheets as new information becomes available. Copies may be made for non-profit use.

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