SAFETY DATA SHEET
EA36 (Eosin/Extra Light Green)
1. IDENTIFICATION
Product Identifiers
Product Name:
EA36 (Eosin/Extra Light Green)
Other Names:
EA36 Stain solution, EA36 Papanicolaou stain
Product Number(s):
AEA36-1L
CAS Number:
-
Recommended use of the chemical and restriction on use
Laboratory use.
Used in hospital and pathology laboratories only.
Company Details
ProSciTech Pty Ltd
11 Carlton Street
KIRWAN QLD 4817
Australia

(07) 4773 9444
www.proscitech.com
Emergency Contact Details
ProSciTech Pty Ltd
11 Carlton Street
KIRWAN QLD 4817
Australia

(07) 4773 9444
www.proscitech.com
2. HAZARDS IDENTIFICATION
Classification of the substance or mixture
Classified as Hazardous according to the Globally Harmonised System of Classification and Labelling of Chemicals (GHS) and Safe Work Australia criteria.
Flam. Liq. 2 H225 Highly flammable liquid and vapour.
Acute Tox. 3 H301 Toxic if swallowed.
Acute Tox. 3 H311 Toxic in contact with skin.
Acute Tox. 3 H331 Toxic if inhaled.
STOT SE 1 H370 Causes damage to organs.
Label Elements
Signal Words
Danger
Hazard Statement(s)
H225 Highly flammable liquid and vapour.
H301+H311+H331 Toxic if swallowed, in contact with skin or if inhaled.
H370 Causes damage to organs.
Precautionary Statement(s)
P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P241 Use explosion-proof electrical/ventilating/lighting/equipment.
P260 Do not breathe dust/fume/gas/mist/vapours/spray.
P280 Wear protective gloves/protective clothing/eye protection/face protection.
P240 Ground/bond container and receiving equipment.
P242 Use only non-sparking tools.
P243 Take precautionary measures against static discharge.
P264 Wash hands thoroughly after handling.
P270 Do not eat, drink or smoke when using this product.
P271 Use only outdoors or in a well-ventilated area.
P301+P310 IF SWALLOWED: Immediately call a POISON CENTER/ doctor. P303+P361+P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower.
P321 Specific treatment (see on this label).
P304+P340 IF INHALED: Remove person to fresh air and keep comfortable for breathing. P312 Call a POISON CENTER/doctor if you feel unwell.
P330 Rinse mouth.
P370+P378 In case of fire: Use for extinction: CO₂, powder or water spray.
P308+P311 IF exposed or concerned: Call a POISON CENTER/doctor.
P361+P364 Take off immediately all contaminated clothing and wash it before reuse. P405 Store locked up.
P403+P233 Store in a well-ventilated place. Keep container tightly closed.
P403+P235 Store in a well-ventilated place. Keep cool.
P501 Dispose of contents/container in accordance with local/regional/national regulations.
Primary route(s) of entry
Not available.
Human Health
Inhalation:
Toxic if inhaled. May cause respiratory irritation, coughing, discomfort, difficulty breathing and shortness of breath.
Ingestion:
Toxic if swallowed. May cause irritation of mouth, throat and stomach, mucous build up, stomach pain, nausea, vomiting and diarrhoea. Overexposure to methanol can cause death or damage to kidneys, liver, lungs, eyes, brain and nervous system.
Eyes:
May cause eye irritation, stinging, tearing and blurred vision.
Skin:
Toxic in contact with skin. Causes skin irritation, itchiness, redness, drying and defatting.
Environment
Not available.
3. COMPOSITION/INFORMATION ON INGREDIENTS
Name
CAS No.
Content (w/w)
Classification
Ethanol
64-17-5
<60%
Flam. Liq. 2, H225
Methanol
67-56-1
<26%
Flam. Liq. 2, H225 Acute Tox. 3, H301 Acute Tox. 3, H311 Acute Tox. 3, H331; STOT SE 1, H370
Acetic acid
64-19-7
0.8%
Flam. Liq. 3, H226Skin Corr. 1A, H314
Disodium 2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoate
17372-87-1
0.6%
Acute Tox. 3, H301 Acute Tox. 3, H311 Acute Tox. 4, H332
Tungstophosphoric acid (H3PW12O40), hydrate
12501-23-4
>0.2%
Skin Corr. 1A, H314 Eye Dam. 1, H318
4. FIRST AID MEASURES
Ingestion
If swallowed, do not induce vomiting. Immediately rinse mouth with water. Give 3 to 4 glasses of water. Never give anything by mouth to an unconscious person. Seek immediate medical attention.
Inhalation
If inhaled, remove to fresh air. If not breathing, give artificial respiration. Do not use mouth-to-mouth method if victim inhaled the substance. Induce artificial respiration with the aid of a pocket mask equipped with a oneway valve or other proper respiratory medical device. If breathing is difficult, give oxygen. Seek medical attention if breathing problems develop.
Skin Contact
In case of skin contact, immediately remove contaminated clothing and wash affected areas with water and soap. Seek medical attention if symptoms occur.
Eye Contact
In case of eye contact, hold eyelids open and rinse with water for at least 15 minutes. Seek immediate medical attention.
Other Information
No further relevant information available.
5. FIREFIGHTING MEASURES
Suitable extinguishing equipment
Water fog, foam, dry chemical or carbon dioxide.
HAZCHEM
Not available.
Special protective equipment and precautions for fire fighters
Hazardous combustion products include acrid smoke and fumes. Product is highly flammable. Vapours may travel considerable distances to a source of ignition where they can ignite, flashback, or explode. Closed containers may explode when exposed to extreme heat. Containers close to fire should be removed if safe to do so. Use water spray to cool fire exposed containers.
Acrid smoke and fumes. When fighting a major fire wear self-contained breathing apparatus and protective equipment.
6. ACCIDENTAL RELEASE MEASURES
Personal precautions, protective equipment and emergency procedures
Wear appropriate respiratory protection and protective clothing. Evacuate all non-essential personnel from affected area. Do not breathe vapours. Ensure adequate ventilation. Extinguish all sources of ignition. Avoid sparks and open flames. No smoking.
Environmental precautions
In the event of a major spill, prevent spillage from entering drains or water courses.
Methods and materials for containment and clean up
Stop leak if safe to do so and absorb spill with sand, earth, vermiculite or some other absorbent material. Collect the spilled material and place into a suitable container for disposal. Use non-sparking tools. For large spills, contact emergency services. Bund the area and blanket the spill with foam or use water fog to disperse vapours.
7. HANDLING AND STORAGE
Precautions for safe handling
Use of safe work practices are recommended to avoid eye or skin contact and inhalation of vapours. Food, beverages and tobacco products should not be stored or consumed where this material is in use. Always wash hands before smoking, eating, drinking or using the toilet. Wash contaminated clothing and other protective equipment before storage or re-use. Provide eyewash fountains and safety showers in close proximity to points of potential exposure.
Conditions for safe storage
Store in a cool, dry and well ventilated area. Keep in original container tightly closed when not in use. Protect from direct sunlight, heat, sparks, open flames and other sources of ignition. Keep away from strong oxidising agents, nitrates, acids and alkalies.
8. EXPOSURE CONTROLS/PERSONAL PROTECTION
Exposure Standards
Material
TWA ppm
TWA mg/m3
STEL ppm
STEL mg/m3
Ethanol
1000ppm
1880 mg/m³
-
-
Methanol
200 ppm
262 mg/m³
250 ppm
328 mg/m³
Acetic acid
10 ppm
25 mg/m³
15 ppm
37 mg/m³
Engineering controls
Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapour below occupational exposure standards.
Prevent accumulation of gases in hollows or sumps. Do not enter room unless monitored by another person (i.e. buddy-buddy system.
Personal protective equipment
Eye and face protection
Eye and face protectors for protection against splashing materials or liquids. See Australian/New Zealand Standard AS/NZS 1337 for more information.
Skin protection
PVC or rubber gloves. See Australian/New Zealand Standard AS/NZS 2161 for more information. When selecting gloves for use against certain chemicals, the degradation resistance, permeation rate and permeation breakthrough time should be considered. Occupational protective clothing (depending on conditions in which it has to be used, in particular as regards the period for which it is worn, which shall be determined on the basis of the seriousness of the risk, the frequency of exposure to the risk, the characteristics of the workstation of each worker and the performance of the protective clothing). See Australian/New Zealand Standard AS/NZS 4501 for more information.
Body protection
No information available.
Respiratory protection
When vapours approach the exposure standards then the use of a half-face organic vapour respirator with dust/mist filter is recommended. When vapours exceed the exposure standards, the use of an positive pressure demand self-contained orairline breathing apparatus is recommended. Filter capacity and respirator type depends on exposure levels. If entering spaces where the airborne concentration of a contaminant is unknown then the use of a selfcontained breathing apparatus (SCBA) with positive pressure air supply is recommended. See Australian Standards AS/NZS 1715 and 1716 for more information.
9. PHYSICAL AND CHEMICAL PROPERTIES
General information
Appearance
Dark reddish green liquid
Odour
Not available.
pH
Not available.
Vapour Pressure
at 20 °C: 128 hPa
Density
0.89 g/cm³
Boiling Point
66 °C
Melting Point
-88 °C
Solubility
Soluble in water.
Specific Gravity of Density
Not available.
Flash Point
13 °C
Flammable (Explosive) Limits
Explosion Limits:
Lower: 5.5 Vol %
Upper: 44 Vol %
Ignition Temperature
Not available.
Formula
Not available.
10. STABILITY AND REACTIVITY
Reactivity
No further relevant information available.
Chemical stability
Stable at ambient temperature and under normal conditions of use.
Possibility of hazardous reactions
Hazardous polymerisation will not occur.
Conditions to avoid
Direct sunlight, heat, sparks, open flames and other sources of ignition.
Incompatible materials
Strong oxidising agents, nitrates, acids and alkalies.
11. TOXICOLOGICAL INFORMATION
Acute effects
Methanol has been reported to cause death or serious irreversible injury such as blindness in humans. Studies in experimental animals indicate that the metabolism of methanol to formic acid results in metabolic acidosis and reversible or irreversible damage to the optic nerve. Ingestion of methanol, even in small amounts, can cause blindness and death. Onset of symptoms may be delayed for 18 - 24 hours and are similar in affect to ethanol poisoning.
Eye contact
May cause eye irritation, stinging, tearing and blurred vision.
Skin contact
Toxic in contact with skin. Causes skin irritation, itchiness, redness, drying and defatting.
Ingestion
Toxic if swallowed. May cause irritation of mouth, throat and stomach, mucous build up, stomach pain, nausea, vomiting and diarrhoea. Overexposure to methanol can cause death or damage to kidneys, liver, lungs, eyes, brain and nervous system.
Inhalation
Toxic if inhaled. May cause respiratory irritation, coughing, discomfort, difficulty breathing and shortness of breath.
Toxicity and irritation
LD??/LC?? Values Relevant for Classification:
64-17-5 Ethanol
Oral LD?? 7060 mg/kg (rat)
Inhalation LC??/4 h 20000 mg/l (rat)
67-56-1 Methanol
Oral LD?? 5628 mg/kg (rat)
Dermal LD?? 15800 mg/kg (rabbit)
Inhalation LC??/4 h 83.8 mg/l (rat)
64-19-7 acetic acid
Oral LD?? 3310 mg/kg (rat)
Dermal LD?? 1060 mg/kg (rabbit)
12. ECOLOGICAL INFORMATION
Ecotoxicity
May cause long term adverse effects in the aquatic environment.
Persistence and degradability
No further relevant information available.
Bioaccumulative potential
No further relevant information available.
Other adverse effects
No further relevant information available.
13. DISPOSAL CONSIDERATIONS
General information
Dispose according to applicable local and state government regulations.
Special Precautions for Landfill or Incineration: Please consult your state Land Waste Management Authority for more information.
14. TRANSPORT INFORMATION
ADG label required
HAZCHEM
Not available.

UN Number
UN1992
Proper shipping name
FLAMMABLE LIQUID, TOXIC, N.O.S.
Transport hazard class
3 F
Packing group
III
Environmental hazard
No information available.
Special precautions for users
Hazchem Code: .3W
Special Provisions: 223, 274
Additional information
Limited Quantities: 5 L
Packagings & IBCs - Packing Instruction: P001, IBC03
Packagings & IBCs - Special Packing Provisions: No information available
Portable Tanks & Bulk Containers - Instructions: T7 Portable Tanks & Bulk Containers - Special Provisions: TP1, TP28
15. REGULATORY INFORMATION
Poisons Schedule Number
6
Other Information
No further relevant information available.
16. OTHER INFORMATION
SDS preparation date
6 October 2021
Comments
This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and shall not establish a legally valid contractual relationship.
This Safety Data Sheet (SDS) has been prepared in compliance with the Preparation of Safety Data Sheets for Hazardous Chemicals Code of Practice February 2016. It is the user's responsibility to determine the suitability of this information for adoption of necessary safety precautions. The information published in this SDS has been compiled from the publications listed in Section 16: to the best of our ability and knowledge these publications are considered accurate. We reserve the right to revise Safety Data Sheets as new information becomes available. Copies may be made for non-profit use.

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