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Cryo Immuno Diamond Knife

Brief informative article on DiATOME cryo immuno diamond knives

cryo immuno diamond knifeDiATOME CRYO IMMUNO DIAMOND KNIVES

The first cryo knife with a diamond platform guarantees the best possible sectioning for cryo-immuno-chemistry.

immuno diamond knife

The diamond platform ensures easy and gentle section collection. Sections are collected directly from the diamond surface using a loop and a sucrose/methylcellulose droplet (Refs. Liou, Peters).
The 35° cutting angle leads to a considerable reduction in mechanical stresses and therefore to improved structure preservation in sucrose-protected samples.

Fusion of late endosome/lyosome with the Mycobacteria tuberculosis phagosome. Aldehyde fixed cryo-section labeled with anti- CD63 and protein-A gold 10nm. Scale bar = 100nm. Nicole van der Wel and Peter J. Peters, Netherlands Cancer Institute, Amsterdam.


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