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Microwave Processing Protocols

Microwave-assisted processing of human colon endoscopic biopsy after normal 10% NFB fix. Microwave processing protocols user note.

microwave processing protocolsMicrowave-assisted processing of human colon endoscopic biopsy after normal 10% NFB fix.
1 x 7 minutes 100% Ethanol @350 Watts TR of 67°C

1 x 5 minutes ACS reagent Grade Isopropanol @350 Watts, TR of 74°C1 x 18 minutes Paraffin @650 Watts, TR of 80°C(400x approximate) H&E.


microwave processed specimenLiver stained with Masson's Trichrome stain using the PELCO HistoWave® (20x). Courtesy of Rick Giberson, Ted Pella, Inc. and Bruce Calkins, Pathology Sciences, Chico.

Microwave Procedure
StepContainer ReagentWattage SettingTemperature RestrictionTime/Pad #
150ml Coplin Jar
xylene
165WNone4 min.
250ml Coplin Jar
100% ETOH
165WNone1 min.
3Wash in tap water
to clear
BENCH STEP
450ml Coplin Jar
Bouin's Solution
315WTR 60°C6 min.
5Wash in tap water
to clear yellow
BENCH STEP
650ml Coplin Jar
Gill #2 Hematoxylin
315WTR 40°C1 min. 20 sec.
750ml Coplin Jar
Wash in tap water to blue
BENCH STEP
850ml Coplin Jar
Biebrich Scarlet - Acid Fuchsin
315WTR 40°C40 sec.
9Rinse in DI water
3 changes
BENCH STEP
1050ml Coplin Jar
Phosphotungstic - Phosphomolybdic Sol
315WTR 40°C1 min.
1150ml Coplin Jar
Analine Blue Sol
165WTR 40°C40 sec.
12Rinse in Tap
then DI water
BENCH STEP
1350ml Coplin Jar
1% Acetic Acid Sol.
BENCH STEP 30 sec.
14Dehydrate through ETOH's clear and mountBENCH STEP

microwave processed specimenIntestine, cut at 4 microns, stained with Mucin Alcian Blue pH2.5 using the PELCO HistoWave® (20x). Courtesy of Rick Giberson, Ted Pella, Inc. and Bruce Calkins, Pathology Sciences, Chico.

Microwave Procedure
StepContainer ReagentWattage SettingTemperature RestrictionTime/Pad #
150ml Coplin Jar
xylene
165WNone4 min.
250ml Coplin Jar
95-100% ETOH
165WNone1 min.
3Wash in tap water
to clear
BENCH STEP
450ml Coplin Jar
3% Acetic Acid
165WTR 45°C30 sec.
550ml Coplin Jar
Alcian Blue Sol.
165WTR 45°C1 min. 30 sec.
6Rinse in DI water
3 changes
BENCH STEP
750ml Coplin Jar
Nuclear Fast Red
165WTR 45°C1 min. 30 sec.
8Rinse in DI water
3 changes
BENCH STEP
9Dehydrate through ETOH's clear and mountBENCH STEP

microwave processed intestinal specimen Intestinal cross-section, cut at 4 microns, stained with PAS using the PELCO HistoWave® (20x). Courtesy of Rick Giberson, Ted Pella, Inc. and Bruce Calkins, Pathology Sciences, Chico.

Microwave Procedure
StepContainer ReagentWattage SettingTemperature RestrictionTime/Pad #
150ml Coplin Jar
xylene
165WNone4 min.
250ml Coplin Jar
95-100% ETOH
165WNone1 min.
3Wash in tap water
to clear
BENCH STEP
450ml Coplin Jar
0.5% Periodic Acid
315WTR 60°C2 min. 30 sec.
5Rinse in DI water
3 changes
BENCH STEP
650ml Coplin Jar
Schiff Reagent
165WTR 45°C2 min.
750ml Coplin Jar
warm tap Water
165WTR 45°C4 min.
8Wash in
tap water
BENCH STEP
950ml Coplin Jar
Gill #1 Hematoxylin
165WTR 45°C40 sec.
10Wash in
DI water
BENCH STEP
11Blue in
Scott's water
BENCH STEP
12Wash in
tap water
BENCH STEP
13Dehydrate through ETOH's clear and mountBENCH STEP
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