Microwave-assisted processing of human colon endoscopic biopsy after normal 10% NFB fix.
1 x 7 minutes 100% Ethanol @350 Watts TR of 67°C
1 x 5 minutes ACS reagent Grade Isopropanol @350 Watts, TR of 74°C1 x 18 minutes Paraffin @650 Watts, TR of 80°C(400x approximate) H&E.
Liver stained with Masson's Trichrome stain using the PELCO HistoWave® (20x). Courtesy of Rick Giberson, Ted Pella, Inc. and Bruce Calkins, Pathology Sciences, Chico.
Microwave Procedure | ||||
Step | Container Reagent | Wattage Setting | Temperature Restriction | Time/Pad # |
1 | 50ml Coplin Jar xylene | 165W | None | 4 min. |
2 | 50ml Coplin Jar 100% ETOH | 165W | None | 1 min. |
3 | Wash in tap water to clear | BENCH STEP | ||
4 | 50ml Coplin Jar Bouin's Solution | 315W | TR 60°C | 6 min. |
5 | Wash in tap water to clear yellow | BENCH STEP | ||
6 | 50ml Coplin Jar Gill #2 Hematoxylin | 315W | TR 40°C | 1 min. 20 sec. |
7 | 50ml Coplin Jar Wash in tap water to blue | BENCH STEP | ||
8 | 50ml Coplin Jar Biebrich Scarlet - Acid Fuchsin | 315W | TR 40°C | 40 sec. |
9 | Rinse in DI water 3 changes | BENCH STEP | ||
10 | 50ml Coplin Jar Phosphotungstic - Phosphomolybdic Sol | 315W | TR 40°C | 1 min. |
11 | 50ml Coplin Jar Analine Blue Sol | 165W | TR 40°C | 40 sec. |
12 | Rinse in Tap then DI water | BENCH STEP | ||
13 | 50ml Coplin Jar 1% Acetic Acid Sol. | BENCH STEP | 30 sec. | |
14 | Dehydrate through ETOH's clear and mount | BENCH STEP |
Intestine, cut at 4 microns, stained with Mucin Alcian Blue pH2.5 using the PELCO HistoWave® (20x). Courtesy of Rick Giberson, Ted Pella, Inc. and Bruce Calkins, Pathology Sciences, Chico.
Microwave Procedure | ||||
Step | Container Reagent | Wattage Setting | Temperature Restriction | Time/Pad # |
1 | 50ml Coplin Jar xylene | 165W | None | 4 min. |
2 | 50ml Coplin Jar 95-100% ETOH | 165W | None | 1 min. |
3 | Wash in tap water to clear | BENCH STEP | ||
4 | 50ml Coplin Jar 3% Acetic Acid | 165W | TR 45°C | 30 sec. |
5 | 50ml Coplin Jar Alcian Blue Sol. | 165W | TR 45°C | 1 min. 30 sec. |
6 | Rinse in DI water 3 changes | BENCH STEP | ||
7 | 50ml Coplin Jar Nuclear Fast Red | 165W | TR 45°C | 1 min. 30 sec. |
8 | Rinse in DI water 3 changes | BENCH STEP | ||
9 | Dehydrate through ETOH's clear and mount | BENCH STEP |
Intestinal cross-section, cut at 4 microns, stained with PAS using the PELCO HistoWave® (20x). Courtesy of Rick Giberson, Ted Pella, Inc. and Bruce Calkins, Pathology Sciences, Chico.
Microwave Procedure | ||||
Step | Container Reagent | Wattage Setting | Temperature Restriction | Time/Pad # |
1 | 50ml Coplin Jar xylene | 165W | None | 4 min. |
2 | 50ml Coplin Jar 95-100% ETOH | 165W | None | 1 min. |
3 | Wash in tap water to clear | BENCH STEP | ||
4 | 50ml Coplin Jar 0.5% Periodic Acid | 315W | TR 60°C | 2 min. 30 sec. |
5 | Rinse in DI water 3 changes | BENCH STEP | ||
6 | 50ml Coplin Jar Schiff Reagent | 165W | TR 45°C | 2 min. |
7 | 50ml Coplin Jar warm tap Water | 165W | TR 45°C | 4 min. |
8 | Wash in tap water | BENCH STEP | ||
9 | 50ml Coplin Jar Gill #1 Hematoxylin | 165W | TR 45°C | 40 sec. |
10 | Wash in DI water | BENCH STEP | ||
11 | Blue in Scott's water | BENCH STEP | ||
12 | Wash in tap water | BENCH STEP | ||
13 | Dehydrate through ETOH's clear and mount | BENCH STEP |