- CHARACTERISTICS OF POLYSTYRENE PARTICLES
- AJUSTING PARTICLE NUMBERS TO REQUIRED CONCENTRATIONS
- VOLUME, NUMBER AND SURFACE AREA VS. PARTICLE SIZE
- AVIDIN POLYSTYRENE PARTICLES (0.8µm)
- ANTIBODY COATED POLYSTYRENE PARTICLES (0.8µm)
- SpheroTECHNICAL NOTES
- SELECTED REFERENCES
LATEX PARTICLES SUSPENSION
Avidin Contents: | ~ 14 µg/mg solid ~ 0.212 nmole/mg solid |
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Binding capacity to Biotin-Fluorescein: | ~ 0.46 nmole/mg solid |
Antibody contents: | ~ 14 µg/mg solid ~ 0.2 µg/sq cm ~1.5x104 IgG/particle |
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Binding capacity to IgG-FITC | ~ 4 µg/mg |
The following Sphero TECHNICAL NOTES are available upon request
- STN-1: Particles Coating Procedures
- STN-2: Determination of Antibody binding to particles
- STN-3: Binding Capacity of Avidin Magnetic Particles
- STN-4: Binding Capacity of Gt-anti-Ms-IgG Magnetic Particles
- STN-5: Binding Capacity of Streptavidin Magnetic Particles
- STN-6: Binding Capacity of Biotin Magnetic Particles
- STN-7: Separation of Mononuclear Cells from Peripheral Blood using SPHERO Gt-anti-Ms-IgG Magnetic Particles
- STN-8: Calibration and Performance Tracking of Flow Cytometer Using SPHERO Calibration Particles
- STN-9: Measuring MESF with Flow Cytometer Using SPHERO Rainbow Calibration Particles.
- STN-10: Magnetic Particles Enzyme Immunoassay (MPEIA) using UltraMag Separator System (UMS-4000)
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Labib, M., Robertson, A., "Application of a Diafiltration Technique in Latex Studies", J. of Colloid and Interfacial Science,, Vol. 67, 543 (1978)
Hechemy, K., Michaelson, E., "Latex Particle Assay in Laboratory Medicine, Part 1", Lab Management, June/July, 27 (1984)
Seaman, G., Goodwin, J., "Physiochemical Factors in Latex Rapid Agglutination Tests", Amer. Clin. Prod. Rev., June, 26 (1986)
Schwenzer, K, MacCrindle, C., "Particle Concentration Fluorescence Immunoassay: Clinical Applications", Clin. Lab Prod.,Sept, 21 (1985)
Jolley, M., Wang, C., Ekenberg, M., Zuelke, M., Kelso, D., "Particle Concentration Fluorescence Immunoassay (PCFIA): A New, Rapid Immunoassay Technique with High Sensitivity", J. Immunol. Methods, Vol. 67, 21 (1984)
Leahy, D., Shah, D., Todd, J., "A Method for Attachment of Peptides to Solid Surface with Enhanced Immunoreactivity", BioTechniques, Vol 13 (5), 738 (1992)
Leahy, D., Shah, D., Arima, T., et al., "Improved Serological Detection of Hepatitis C Virus with a Paramagnetic Microparticles Assay using Multiple Antigenic Sequences", Transfusion, Vol. 32 (6), 548 (1992)
Todd, J., Kink, J., Shah, D., et al., "A novel Semi-automated Paramagnetic Microparticle Based Enzyme Immunoassay for Hepatitis C Virus: Its application to serological testing", J. of Immunoassay, Vol. 13 (3), 393 (1992)
Phillips, D., Reimer, C., Wells, T., Black, C., "Quantitative Characterization of Specificity and Potency of Conjugated Antibody with Solid-Phase, Antigen Bead Standards", J. of Immunol. Methods, Vol. 34 315 (1980)
Kreuter, J., Berg, U., Liehl, E., Soliva, M., Speiser, P., "Influence of the Particle Size on the Adjuvant Effect of Particulate Polymeric Adjuvants", Vaccine, Vol. 4, June, 125 (1986)
Hadfield, S., Lane, A., McIllmurray, M., "A Novel Coloured Latex Test for the Detection and Identification of more than one Antigen", J. of Immunol. Methods, Vol. 97, 153 (1987)
Staros, J., Wright, R., Swingle, D., "Enhancement by N-Hydroxysulfo-Succinimide of Water-Soluble Carbodiimide Mediated Coupling Reagents", Anal. Chem. Vol. 156, 220 (1986)
Van den Hul, H. Vanderhoff, J., "The Characterization of Latex Particle Surface by Ion Exchange and Conductiometric Titration", J Electroanal. Chem. Vol. 37, 161 (1972)
Andrew J. Beavis and Kenneth J. Pennline, "Detection of Cell-Surface Antigens Using Antibody-Conjugated Fluorospheres (ACF): Application for Six-Colour Immunofluorescence", BioTechniques 21:498-503 (September 1996)